Genotyping and primer details


Genotyping and primer details for 875C10 - At4g21100
Line ID 875C10
Locus At4g21100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11259433 of line 875C10 Chr4:11259433
   
Insertion allele amplicon 8474 - 83ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 83ra: 5'-TTCAGTTGTATAAGTGGATGCTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11259775
   
wt amplicon (over InsPos) 06si - 83ra
wt amplicon start Chr4:11258868
wt amplicon end Chr4:11259775
wt amplicon size 908 bp
Locus specific primer 83ra: 5'-TTCAGTTGTATAAGTGGATGCTGC-3'
2nd locus specific primer 06si: 5'-GTCGAGAAAGTAACAAACGAAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11