Genotyping and primer details


Genotyping and primer details for 199G03 - At4g26488
Line ID 199G03
Locus At4g26488 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr: of line 199G03 Chr:
   
wt amplicon (over InsPos) 13ua - 15qv
wt amplicon start Chr4:13380100
wt amplicon end Chr4:13380719
wt amplicon size 620 bp
Locus specific primer 15qv: 5'-ATCTGTCCCCTGTAATCAATCAAA-3'
2nd locus specific primer 13ua: 5'-AAAGGCTGAACACAAAACAGAAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11