Genotyping and primer details


Genotyping and primer details for 447C01 - At4g27240
Line ID 447C01
Locus At4g27240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13640697 of line 447C01 Chr4:13640697
   
Insertion allele amplicon 8474 - 15wu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15wu: 5'-ACGATACAGAGAGAAAACACCGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13640205
   
wt amplicon (over InsPos) 23xd - 15wu
wt amplicon start Chr4:13640205
wt amplicon end Chr4:13641735
wt amplicon size 1531 bp
Locus specific primer 15wu: 5'-ACGATACAGAGAGAAAACACCGAT-3'
2nd locus specific primer 23xd: 5'-TGAAAGCTAAGATTTTTACGACTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11