Genotyping and primer details


Genotyping and primer details for 401E04 - At4g31170
Line ID 401E04
Locus At4g31170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15155129 of line 401E04 Chr4:15155129
   
Insertion allele amplicon 8474 - 20uk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 20uk: 5'-AGATGATGAAGACGAAGATGACCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15155997
   
Insertion allele amplicon 8474 - 21uk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21uk: 5'-TAATTGTTGTCGTTGTTGTGATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15154785
   
wt amplicon (over InsPos) 06ul - 29tv
wt amplicon start Chr4:15154764
wt amplicon end Chr4:15155752
wt amplicon size 989 bp
Locus specific primer 29tv: 5'-CAAAGGACGGACCAAAATTAAATA-3'
2nd locus specific primer 06ul: 5'-AAGTCTTGGGTGAAGGCATAGTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11