Genotyping and primer details


Genotyping and primer details for 394B09 - At4g31750
Line ID 394B09
Locus At4g31750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15365118 of line 394B09 Chr4:15365118
   
Insertion allele amplicon 3144 - 35ah
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 35ah: 5'-GGTACGATACCATGATCTATTGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15364688
   
Insertion allele amplicon 8474 - AB57
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AB57: 5'-ATGAAATTTGCAATAAGTCGCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15365600
   
wt amplicon (over InsPos) AB57 - 35ah
wt amplicon start Chr4:15364688
wt amplicon end Chr4:15365600
wt amplicon size 913 bp
Locus specific primer 35ah: 5'-GGTACGATACCATGATCTATTGGG-3'
2nd locus specific primer AB57: 5'-ATGAAATTTGCAATAAGTCGCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11