Genotyping and primer details


Genotyping and primer details for 785E02 - At4g31810
Line ID 785E02
Locus At4g31810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15388848 of line 785E02 Chr4:15388848
   
Insertion allele amplicon 8474 - 49kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 49kh: 5'-CCAAGGAACGTATTTAAAACCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15389373
   
wt amplicon (over InsPos) 07mf - 49kh
wt amplicon start Chr4:15388245
wt amplicon end Chr4:15389373
wt amplicon size 1129 bp
Locus specific primer 49kh: 5'-CCAAGGAACGTATTTAAAACCAAA-3'
2nd locus specific primer 07mf: 5'-ATTTACTTGTGCAGTACGCTGCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11