Genotyping and primer details


Genotyping and primer details for 903G11 - At4g34660
Line ID 903G11
Locus At4g34660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16548508 of line 903G11 Chr4:16548508
   
Insertion allele amplicon 8474 - 14wl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14wl: 5'-AGCTTGTTTTCTAATTGCATCCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16548271
   
Insertion allele amplicon 8474 - 28wm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 28wm: 5'-CAATCTCACATCGAATCAACGTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16548002
   
Insertion allele amplicon 8474 - 51yc
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 51yc: 5'-GGTTGTGAAATCCATACCCTACAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16549566
   
Insertion allele amplicon 8474 - 61xk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 61xk: 5'-CTTGGAACATGTAGCACCAATATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16547859
   
Insertion allele amplicon 8474 - 80ns
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 80ns: 5'-TACAGAGACTGGAGAAACAAGCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16548787
   
Insertion allele amplicon 8474 - 81ns
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 81ns: 5'-AGAACTTTAACCTGCTGCTGTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16548224
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11