Genotyping and primer details


Genotyping and primer details for 170C01 - At5g04470
Line ID 170C01
Locus At5g04470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1267383 of line 170C01 Chr5:1267383
   
Insertion allele amplicon 8474 - MZ54
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MZ54: 5'-TCATCATAACCCATGACACAAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1266425
   
wt amplicon (over InsPos) 35au - MZ54
wt amplicon start Chr5:1266425
wt amplicon end Chr5:1267427
wt amplicon size 1003 bp
Locus specific primer MZ54: 5'-TCATCATAACCCATGACACAAAAT-3'
2nd locus specific primer 35au: 5'-GGCATGTATGTAAAGACAGAAGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11