Genotyping and primer details


Genotyping and primer details for 929A02 - At5g06780
Line ID 929A02
Locus At5g06780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2096582 of line 929A02 Chr5:2096582
   
Insertion allele amplicon 8474 - 45qz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 45qz: 5'-CCAATCAGCTCTTTATAGCCTCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2096920
   
wt amplicon (over InsPos) 50si - 45qz
wt amplicon start Chr5:2095756
wt amplicon end Chr5:2096920
wt amplicon size 1165 bp
Locus specific primer 45qz: 5'-CCAATCAGCTCTTTATAGCCTCTG-3'
2nd locus specific primer 50si: 5'-AGTAGAACCAATCGACGGATACTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11