Genotyping and primer details


Genotyping and primer details for 894G04 - At5g14490
Line ID 894G04
Locus At5g14490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4671669 of line 894G04 Chr5:4671669
   
Insertion allele amplicon 8474 - 30kk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 30kk: 5'-AGCAAAAACCAAACACTGAAAACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4672271
   
wt amplicon (over InsPos) 35sq - 30kk
wt amplicon start Chr5:4671172
wt amplicon end Chr5:4672271
wt amplicon size 1100 bp
Locus specific primer 30kk: 5'-AGCAAAAACCAAACACTGAAAACT-3'
2nd locus specific primer 35sq: 5'-GTACTAGTTTTCGGTGTGCTAGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11