Genotyping and primer details


Genotyping and primer details for 291C10 - At5g14880
Line ID 291C10
Locus At5g14880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4814693 of line 291C10 Chr5:4814693
   
Insertion allele amplicon 8474 - SD41
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer SD41: 5'-ATCGCATCTTTTGAAGTTTCTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4814173
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11