Genotyping and primer details


Genotyping and primer details for 102C06 - At5g15470
Line ID 102C06
Locus At5g15470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5023496 of line 102C06 Chr5:5023496
   
Insertion allele amplicon 8474 - 03kn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03kn: 5'-CTGTTGCCTAATTCAAGGATTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5023905
   
wt amplicon (over InsPos) 09le - 03kn
wt amplicon start Chr5:5022931
wt amplicon end Chr5:5023905
wt amplicon size 975 bp
Locus specific primer 03kn: 5'-CTGTTGCCTAATTCAAGGATTCTC-3'
2nd locus specific primer 09le: 5'-AAGAGCCACAGATAGATGCAAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11