Genotyping and primer details


Genotyping and primer details for 800C02 - At5g22060
Line ID 800C02
Locus At5g22060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7305326 of line 800C02 Chr5:7305326
   
Insertion allele amplicon 8474 - 50kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 50kh: 5'-GAAGCAAAGTCTTCAAAACCAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7305790
   
wt amplicon (over InsPos) 15ky - 50kh
wt amplicon start Chr5:7304897
wt amplicon end Chr5:7305790
wt amplicon size 894 bp
Locus specific primer 50kh: 5'-GAAGCAAAGTCTTCAAAACCAAAC-3'
2nd locus specific primer 15ky: 5'-AGCCTTCCTAGAAAGTGAGAGCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11