Genotyping and primer details


Genotyping and primer details for 052G08 - At5g22940
Line ID 052G08
Locus At5g22940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7677366 of line 052G08 Chr5:7677366
   
Insertion allele amplicon 8474 - MK63
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MK63: 5'-TTAAACATGAGGACTTTGGAGCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7677984
   
wt amplicon (over InsPos) MK63 - CT41
wt amplicon start Chr5:7676862
wt amplicon end Chr5:7677984
wt amplicon size 1123 bp
Locus specific primer CT41: 5'-ACAAGGGCATTATAGTCATTCCAC-3'
2nd locus specific primer MK63: 5'-TTAAACATGAGGACTTTGGAGCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11