Genotyping and primer details


Genotyping and primer details for 431G08 - At5g36940
Line ID 431G08
Locus At5g36940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:14591193 of line 431G08 Chr5:14591193
   
Insertion allele amplicon 8474 - 56ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 56ra: 5'-ACCCCGTAAGGAAAATACCTAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:14591975
   
wt amplicon (over InsPos) 34rt - 56ra
wt amplicon start Chr5:14591150
wt amplicon end Chr5:14591975
wt amplicon size 826 bp
Locus specific primer 56ra: 5'-ACCCCGTAAGGAAAATACCTAAGA-3'
2nd locus specific primer 34rt: 5'-TTGATATCTTCCCTTGCTTGAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11