Genotyping and primer details


Genotyping and primer details for 236G04 - At5g39170
Line ID 236G04
Locus At5g39170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:15681137 of line 236G04 Chr5:15681137
   
Insertion allele amplicon 8474 - 01jc
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01jc: 5'-CCATAGTTTCTCCAAGATGTATAAATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:15681341
   
Insertion allele amplicon 8474 - 18hm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18hm: 5'-ACACTATACAGTTGCCTAGGTCCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:15668370
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11