Genotyping and primer details


Genotyping and primer details for 011A11 - At5g50420
Line ID 011A11
Locus At5g50420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20531696 of line 011A11 Chr5:20531696
   
Insertion allele amplicon 8474 - 01hd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01hd: 5'-GAAAGCAAGATGAAAAAGCCATAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20531338
   
wt amplicon (over InsPos) 11ig - 01hd
wt amplicon start Chr5:20531338
wt amplicon end Chr5:20532198
wt amplicon size 861 bp
Locus specific primer 01hd: 5'-GAAAGCAAGATGAAAAAGCCATAC-3'
2nd locus specific primer 11ig: 5'-GTCTGTTTTGTTTGAGGATGTGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11