Genotyping and primer details


Genotyping and primer details for 926H03 - At5g53320
Line ID 926H03
Locus At5g53320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21637077 of line 926H03 Chr5:21637077
   
Insertion allele amplicon 8474 - CN34
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CN34: 5'-CATCTCCACCATCTCTTCCTCTAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21636636
   
Insertion allele amplicon 8474 - EU96
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU96: 5'-ATCTTCAAAGGACAAACCTAGCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21637537
   
wt amplicon (over InsPos) CN34 - EU96
wt amplicon start Chr5:21636636
wt amplicon end Chr5:21637537
wt amplicon size 902 bp
Locus specific primer EU96: 5'-ATCTTCAAAGGACAAACCTAGCAA-3'
2nd locus specific primer CN34: 5'-CATCTCCACCATCTCTTCCTCTAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11