Genotyping and primer details


Genotyping and primer details for 757B03 - At5g60320
Line ID 757B03
Locus At5g60320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24271218 of line 757B03 Chr5:24271218
   
Insertion allele amplicon 8474 - BH04
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BH04: 5'-GATGAGGAATCTGAGGAAGTTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24271654
   
Insertion allele amplicon 8474 - EU56
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU56: 5'-GAAACTCCAGAAACAAAACCAGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24270764
   
wt amplicon (over InsPos) BH04 - EU56
wt amplicon start Chr5:24270764
wt amplicon end Chr5:24271654
wt amplicon size 891 bp
Locus specific primer EU56: 5'-GAAACTCCAGAAACAAAACCAGAC-3'
2nd locus specific primer BH04: 5'-GATGAGGAATCTGAGGAAGTTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11