Genotyping and primer details


Genotyping and primer details for 374C08 - At5g67610
Line ID 374C08
Locus At5g67610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26962971 of line 374C08 Chr5:26962971
   
Insertion allele amplicon 8474 - 24ni
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 24ni: 5'-ATATCCATGGACTTGGAAGGTTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26963236
   
wt amplicon (over InsPos) 76wb - 24ni
wt amplicon start Chr5:26962340
wt amplicon end Chr5:26963236
wt amplicon size 897 bp
Locus specific primer 24ni: 5'-ATATCCATGGACTTGGAAGGTTTA-3'
2nd locus specific primer 76wb: 5'-CCATCTCTGTTAGCAGACTTTCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11