Genotyping and primer details


Genotyping and primer details for 968G05 - F24K9
Line ID 968G05
Locus F24K9 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3644276 of line 968G05 Chr3:3644276
   
wt amplicon (over InsPos) 24xg - 31wm
wt amplicon start Chr3:3643676
wt amplicon end Chr3:3644761
wt amplicon size 1086 bp
Locus specific primer 31wm: 5'-GTGGCTTCTCTTAGCTTTTGAAGA-3'
2nd locus specific primer 24xg: 5'-CGGTATTTTAAAGGGAAAGACACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11