Genotyping and primer details


Genotyping and primer details for 358A06 - T1B9
Line ID 358A06
Locus T1B9 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2260508 of line 358A06 Chr3:2260508
   
Insertion allele amplicon 8474 - 15nz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15nz: 5'-TTTCAGTTTGCAGAGACCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23296620
   
Insertion allele amplicon 8474 - 82wk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 82wk: 5'-ATAATCGGATTCAGTGATAAACGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2261279
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11