Genotyping and primer details

Genotyping and primer details for 001A08 - At4g16500
Line ID 001A08
Locus At4g16500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9301871 of line 001A08 Chr4:9301871
Insertion allele amplicon 8474 - AY73
Locus specific primer AY73: 5'-AAACCATTTGTAACTTGGTTTTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9302483
wt amplicon (over InsPos) AY74 - AY73
wt amplicon start Chr4:9301438
wt amplicon end Chr4:9302483
wt amplicon size 1046 bp
Locus specific primer AY73: 5'-AAACCATTTGTAACTTGGTTTTCG-3'
2nd locus specific primer AY74: 5'-AACAACAACACAAACGTCAGAGTC-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11