Genotyping and primer details


Genotyping and primer details for 004D08 - At3g24463
Line ID 004D08
Locus At3g24463 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8887800 of line 004D08 Chr3:8887800
   
wt amplicon (over InsPos) 01qm - 21do
wt amplicon start Chr3:8887261
wt amplicon end Chr3:8888424
wt amplicon size 1164 bp
Locus specific primer 21do: 5'-TCCAGCTCTTATGGGACTCTATGT-3'
2nd locus specific primer 01qm: 5'-AAACCGACTTGGAACACATAATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11