Genotyping and primer details


Genotyping and primer details for 008F10 - At1g14280
Line ID 008F10
Locus At1g14280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4878002 of line 008F10 Chr1:4878002
   
Insertion allele amplicon 8474 - CF05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CF05: 5'-AATTTGTAATTTTGTTCCTTGGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4877398
   
wt amplicon (over InsPos) CT31 - CF05
wt amplicon start Chr1:4877398
wt amplicon end Chr1:4878433
wt amplicon size 1036 bp
Locus specific primer CF05: 5'-AATTTGTAATTTTGTTCCTTGGGA-3'
2nd locus specific primer CT31: 5'-CCATGGAAATTTCTTCTGAATGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11