Genotyping and primer details


Genotyping and primer details for 008G02 - At2g25820
Line ID 008G02
Locus At2g25820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11015571 of line 008G02 Chr2:11015571
   
Insertion allele amplicon 8474 - MR93
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MR93: 5'-AAATGTCTCATTCCTTCTTGCCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11014892
   
wt amplicon (over InsPos) 07si - MR93
wt amplicon start Chr2:11014892
wt amplicon end Chr2:11016017
wt amplicon size 1126 bp
Locus specific primer MR93: 5'-AAATGTCTCATTCCTTCTTGCCTA-3'
2nd locus specific primer 07si: 5'-GGTACACACTTTTCCTCCGTATGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11