Genotyping and primer details


Genotyping and primer details for 009B03 - At1g43650
Line ID 009B03
Locus At1g43650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:16445108 of line 009B03 Chr1:16445108
   
Insertion allele amplicon 8474 - DC30
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DC30: 5'-TCACCTTGCTCTAACAACACAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:16444504
   
wt amplicon (over InsPos) FP38 - DC30
wt amplicon start Chr1:16444504
wt amplicon end Chr1:16445683
wt amplicon size 1180 bp
Locus specific primer DC30: 5'-TCACCTTGCTCTAACAACACAATC-3'
2nd locus specific primer FP38: 5'-TGAAAAGATTAGAGACGGTGACCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11