Genotyping and primer details


Genotyping and primer details for 010G07 - At3g05940
Line ID 010G07
Locus At3g05940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1778997 of line 010G07 Chr3:1778997
   
Insertion allele amplicon 8474 - DC31
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DC31: 5'-GAAAAATCACCTGCCAATAGGTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1778382
   
Insertion allele amplicon 3144 - FP39
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FP39: 5'-AGCAGTTCCAAGAGTTTTCTTCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1779483
   
wt amplicon (over InsPos) FP39 - DC31
wt amplicon start Chr3:1778382
wt amplicon end Chr3:1779483
wt amplicon size 1102 bp
Locus specific primer DC31: 5'-GAAAAATCACCTGCCAATAGGTTA-3'
2nd locus specific primer FP39: 5'-AGCAGTTCCAAGAGTTTTCTTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11