Genotyping and primer details


Genotyping and primer details for 012H09 - At5g52260
Line ID 012H09
Locus At5g52260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21219950 of line 012H09 Chr5:21219950
   
Insertion allele amplicon 8474 - 63yl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 63yl: 5'-GGCAAATATAAAAAGAAATAACGTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21218795
   
Insertion allele amplicon 8474 - M19a
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer M19a: 5'-ATTTTGCAATCCGAGACCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21220699
   
wt amplicon (over InsPos) 01ia - M19a
wt amplicon start Chr5:21219442
wt amplicon end Chr5:21220699
wt amplicon size 1258 bp
Locus specific primer M19a: 5'-ATTTTGCAATCCGAGACCAC-3'
2nd locus specific primer 01ia: 5'-TTGACATCAATCTGTTTGACATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11