Genotyping and primer details


Genotyping and primer details for 022F04 - At3g28910
Line ID 022F04
Locus At3g28910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:10912016 of line 022F04 Chr3:10912016
   
Insertion allele amplicon 3144 - FL34
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FL34: 5'-GGTTTGGATTGAAATTTGTGTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:10911687
   
Insertion allele amplicon 8474 - RS07
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RS07: 5'-CACTTCTCAAACAAAGACCATTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:10912789
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11