Genotyping and primer details


Genotyping and primer details for 025H06 - At4g36860
Line ID 025H06
Locus At4g36860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17359850 of line 025H06 Chr4:17359850
   
Insertion allele amplicon 8474 - 06by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06by: 5'-TCCTCATTCCTTCTAGTCACAGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17360461
   
wt amplicon (over InsPos) 33oz - 06by
wt amplicon start Chr4:17360461
wt amplicon end Chr:?
wt amplicon size 1035 bp
Locus specific primer 06by: 5'-TCCTCATTCCTTCTAGTCACAGGT-3'
2nd locus specific primer 33oz: unknown
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11