Genotyping and primer details


Genotyping and primer details for 050B11 - At5g64610
Line ID 050B11
Locus At5g64610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25828526 of line 050B11 Chr5:25828526
   
Insertion allele amplicon 8474 - IL19
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IL19: 5'-TTTACCATCCAGACAAAAGATTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25828191
   
wt amplicon (over InsPos) FQ13 - IL19
wt amplicon start Chr5:25828191
wt amplicon end Chr5:25828852
wt amplicon size 662 bp
Locus specific primer IL19: 5'-TTTACCATCCAGACAAAAGATTCC-3'
2nd locus specific primer FQ13: 5'-GCCTTCTGTGAGTTTTTGTTTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11