Genotyping and primer details


Genotyping and primer details for 053C12 - At5g53530
Line ID 053C12
Locus At5g53530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21746895 of line 053C12 Chr5:21746895
   
Insertion allele amplicon 8474 - MJ63
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MJ63: 5'-TATTAGCTCCTGCTCCAGTTGATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21746600
   
wt amplicon (over InsPos) 29rd - MJ63
wt amplicon start Chr5:21746600
wt amplicon end Chr5:21747327
wt amplicon size 728 bp
Locus specific primer MJ63: 5'-TATTAGCTCCTGCTCCAGTTGATT-3'
2nd locus specific primer 29rd: 5'-ATATGAGACATACAATGGCGTGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11