Genotyping and primer details


Genotyping and primer details for 057B09 - At1g05830
Line ID 057B09
Locus At1g05830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1757192 of line 057B09 Chr1:1757192
   
Insertion allele amplicon 8474 - 09bd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09bd: 5'-GAAGGATGGATCAACTTCAGAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1756588
   
Insertion allele amplicon 8474 - IN40
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IN40: 5'-GAACTATATTTGCGCTGAGAAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1757786
   
wt amplicon (over InsPos) 09bd - IN40
wt amplicon start Chr1:1756588
wt amplicon end Chr1:1757786
wt amplicon size 1199 bp
Locus specific primer IN40: 5'-GAACTATATTTGCGCTGAGAAACC-3'
2nd locus specific primer 09bd: 5'-GAAGGATGGATCAACTTCAGAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11