Genotyping and primer details


Genotyping and primer details for 058G08 - At5g59730
Line ID 058G08
Locus At5g59730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24065861 of line 058G08 Chr5:24065861
   
Insertion allele amplicon 3144 - 04bd
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 04bd: 5'-ATTCCTCCACCTTCCTCATCTTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24066187
   
Insertion allele amplicon 8474 - DN40
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DN40: 5'-AAACCAGAGGAAGAGAAAACATGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24065232
   
wt amplicon (over InsPos) 04bd - DN40
wt amplicon start Chr5:24065232
wt amplicon end Chr5:24066187
wt amplicon size 956 bp
Locus specific primer DN40: 5'-AAACCAGAGGAAGAGAAAACATGA-3'
2nd locus specific primer 04bd: 5'-ATTCCTCCACCTTCCTCATCTTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11