Genotyping and primer details


Genotyping and primer details for 062D03 - At5g67540
Line ID 062D03
Locus At5g67540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26945386 of line 062D03 Chr5:26945386
   
Insertion allele amplicon 8474 - LJ01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer LJ01: 5'-TGCTTCGTATTCTTAGGGTTAAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26945970
   
wt amplicon (over InsPos) 01vv - LJ01
wt amplicon start Chr5:26944663
wt amplicon end Chr5:26945970
wt amplicon size 1308 bp
Locus specific primer LJ01: 5'-TGCTTCGTATTCTTAGGGTTAAGG-3'
2nd locus specific primer 01vv: 5'-TTACTCTCTTCATAACCGGTGTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11