Genotyping and primer details


Genotyping and primer details for 071F07 - At4g26890
Line ID 071F07
Locus At4g26890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13513263 of line 071F07 Chr4:13513263
   
Insertion allele amplicon 8474 - AL05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AL05: 5'-AGGAAGATCAAAAACAGAGATGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13512775
   
Insertion allele amplicon 8474 - DL36
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DL36: 5'-GTCAAAGTAGGAATGTTCTGCTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13513963
   
wt amplicon (over InsPos) AL05 - DL36
wt amplicon start Chr4:13512775
wt amplicon end Chr4:13513963
wt amplicon size 1189 bp
Locus specific primer DL36: 5'-GTCAAAGTAGGAATGTTCTGCTCC-3'
2nd locus specific primer AL05: 5'-AGGAAGATCAAAAACAGAGATGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11