Genotyping and primer details


Genotyping and primer details for 086H03 - At5g17167
Line ID 086H03
Locus At5g17167 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5645493 of line 086H03 Chr5:5645493
   
wt amplicon (over InsPos) 19tl - 15so
wt amplicon start Chr5:5645012
wt amplicon end Chr5:5646529
wt amplicon size 1518 bp
Locus specific primer 15so: 5'-AGCTTTAGGACAAGCTGAA-3'
2nd locus specific primer 19tl: 5'-CAATGGAAATCTCTCTCTGTTTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11