Genotyping and primer details


Genotyping and primer details for 087B10 - At3g16470
Line ID 087B10
Locus At3g16470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5596337 of line 087B10 Chr3:5596337
   
Insertion allele amplicon 8474 - 13wh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13wh: 5'-CATTCAAAGGAAAAACTTCTCAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5596784
   
Insertion allele amplicon 8474 - 85wh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 85wh: 5'-CGAGAAACTTTTCGGGTCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5596837
   
Insertion allele amplicon 8474 - 86wh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 86wh: 5'-CAATGAGAGTTCTCTTGTCATCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5597498
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11