Genotyping and primer details


Genotyping and primer details for 087C09 - At2g36300
Line ID 087C09
Locus At2g36300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15213590 of line 087C09 Chr2:15213590
   
Insertion allele amplicon 8474 - LB31
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer LB31: 5'-ATCTCTTTGCTGATTTGGTGAGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15214171
   
wt amplicon (over InsPos) 02li - LB31
wt amplicon start Chr2:15213068
wt amplicon end Chr2:15214171
wt amplicon size 1104 bp
Locus specific primer LB31: 5'-ATCTCTTTGCTGATTTGGTGAGAT-3'
2nd locus specific primer 02li: 5'-GCAACATCAATTCCAATACTCTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11