Genotyping and primer details


Genotyping and primer details for 089C04 - At4g34850
Line ID 089C04
Locus At4g34850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16609438 of line 089C04 Chr4:16609438
   
Insertion allele amplicon 8474 - PH06
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PH06: 5'-CTACTTCAAAACCACCAAGTGTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16608474
   
wt amplicon (over InsPos) 07cr - PH06
wt amplicon start Chr4:16608474
wt amplicon end Chr4:16609612
wt amplicon size 1139 bp
Locus specific primer PH06: 5'-CTACTTCAAAACCACCAAGTGTGA-3'
2nd locus specific primer 07cr: 5'-GCTCTCCTCCAACATATACTCCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11