Genotyping and primer details


Genotyping and primer details for 103B07 - At5g23690
Line ID 103B07
Locus At5g23690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7987816 of line 103B07 Chr5:7987816
   
Insertion allele amplicon 8474 - FT08
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FT08: 5'-TCATTGATTTATTGTTGGCGTTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7987367
   
Insertion allele amplicon 3144 - FT09
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FT09: 5'-GACTTTGAAACTACCAACCTTTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7988156
   
wt amplicon (over InsPos) FT09 - FT08
wt amplicon start Chr5:7987367
wt amplicon end Chr5:7988156
wt amplicon size 790 bp
Locus specific primer FT08: 5'-TCATTGATTTATTGTTGGCGTTTA-3'
2nd locus specific primer FT09: 5'-GACTTTGAAACTACCAACCTTTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11