Genotyping and primer details


Genotyping and primer details for 105D10 - At5g50430
Line ID 105D10
Locus At5g50430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20535817 of line 105D10 Chr5:20535817
   
Insertion allele amplicon 8474 - JM02
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JM02: 5'-TGACTTAAGCAGGATGGTTTCTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20536470
   
wt amplicon (over InsPos) 02uo - JM02
wt amplicon start Chr5:20535263
wt amplicon end Chr5:20536470
wt amplicon size 1208 bp
Locus specific primer JM02: 5'-TGACTTAAGCAGGATGGTTTCTTT-3'
2nd locus specific primer 02uo: 5'-AGGAATGAAAATGAAGACTGTGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11