Genotyping and primer details


Genotyping and primer details for 105E10 - At2g30420
Line ID 105E10
Locus At2g30420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12961122 of line 105E10 Chr2:12961122
   
Insertion allele amplicon 8474 - IN12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IN12: 5'-AAAAATTCACATGGCGAGAGTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12961530
   
wt amplicon (over InsPos) 17fo - IN12
wt amplicon start Chr2:12960669
wt amplicon end Chr2:12961530
wt amplicon size 862 bp
Locus specific primer IN12: 5'-AAAAATTCACATGGCGAGAGTAAC-3'
2nd locus specific primer 17fo: 5'-TTTGGTGATTGTTCTTTCATTCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11