Genotyping and primer details


Genotyping and primer details for 106A05 - At1g28140
Line ID 106A05
Locus At1g28140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:9833114 of line 106A05 Chr1:9833114
   
Insertion allele amplicon 3144 - RK68
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer RK68: 5'-CTGAGGGGCAAAATATCAAAAATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9832700
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11