Genotyping and primer details


Genotyping and primer details for 108E01 - At2g26660
Line ID 108E01
Locus At2g26660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11340852 of line 108E01 Chr2:11340852
   
Insertion allele amplicon 3144 - MM02
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer MM02: 5'-GCCTTATCTAAGCCCACATAAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11341187
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11