Genotyping and primer details

Genotyping and primer details for 110C11 - At1g20620
Line ID 110C11
Locus At1g20620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7144070 of line 110C11 Chr1:7144070
Insertion allele amplicon 8474 - ZC01
Locus specific primer ZC01: 5'-TGTAAAACTTGACAGCAAAACCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7144728
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 02.09.2016 15:25