Genotyping and primer details

Genotyping and primer details for 119E08 - At3g06960
Line ID 119E08
Locus At3g06960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2194956 of line 119E08 Chr3:2194956
Insertion allele amplicon 3144 - 02hz
Locus specific primer 02hz: 5'-AATACTTCAGGGCAGATTTTCTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2194705
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11