Genotyping and primer details


Genotyping and primer details for 130F06 - At5g10570
Line ID 130F06
Locus At5g10570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3342600 of line 130F06 Chr5:3342600
   
Insertion allele amplicon 8474 - H61a
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer H61a: 5'-CTCCGATCCATTGTCCCTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3341864
   
wt amplicon (over InsPos) 40wn - H61a
wt amplicon start Chr5:3341864
wt amplicon end Chr5:3343224
wt amplicon size 1361 bp
Locus specific primer H61a: 5'-CTCCGATCCATTGTCCCTAA-3'
2nd locus specific primer 40wn: 5'-AGACGCAGCTATTTAAAGAGGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 02.09.2016 15:25