Genotyping and primer details


Genotyping and primer details for 132C08 - MCK7
Line ID 132C08
Locus MCK7 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23584471 of line 132C08 Chr5:23584471
   
wt amplicon (over InsPos) 01nd - 02md
wt amplicon start Chr5:23583665
wt amplicon end Chr5:23584708
wt amplicon size 1044 bp
Locus specific primer 02md: 5'-TAGTCAATTTTCCCTCAAGATGCT-3'
2nd locus specific primer 01nd: 5'-GAGAGCTCCCATCATATTACTTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11